Sorry for not making the problem clear! The overall B factor for the 3.0 A structure is around 98.00 A*2. Most of the deposited structures in the PDB site around this resolution has an av B around 70 A*2.... All other statistics looks fine. I got a warning message while trying to upload the structure in PDB that 98 was higher than the norm! The reason why the structure has such a high B factor could be due to disordered loops... All the same I was wondering how acceptable this structure would be to picky reviewers.. And 'better the B factors' means bring them down...sorry couldn't phrase it 'better'! I am going to try doing refinement with group B factors! Would that help? Thanks Robbie, I think this I need to make the best of the model I have. Prof. Sekar will try PDB_redo..Thanks!
On Thu, Oct 13, 2016 at 11:41 AM, Pavel Afonine <pafon...@gmail.com> wrote: > I fully agree with Dale in not understanding what the problem is. Perhaps > I have a better chance if you clearly explain what exactly you mean by "is > there any way to better the B factors". > Pavel > > > On Thu, Oct 13, 2016 at 12:57 PM, Dale Tronrud <de...@daletronrud.com> > wrote: > >> I'm sorry but I don't understand what your problem is. Do you think >> the B factors are too small for a 3A data set? A range of 70 to 75 is a >> little smaller than usual but probably not out of bounds. >> >> Dale Tronrud >> >> On 10/12/2016 7:59 PM, sunanda williams wrote: >> > Hi all, >> > I have a structure at 3.0 A and R/Rfree of 24/28. The mean B value is >> > around 98. The B value is especially high at the N terminus and two loop >> > regions (around 120-150 AA). >> > The rest of the structure averaged around 70-75. Has anyone faced such a >> > scenario? How reliable is the structure and is there any way to better >> > the B factors. >> > Any help is appreciated. >> > Thank you!! >> > >
