...and there is a residue missing (159).

B

It seems your chain ID is different, so it would not connect two
different chain ID covalently. So please change your chain ID and try to
connect.


Good luck

On Sat, May 23, 2015 at 3:53 PM, Ishan Rathore <ishanrathor...@gmail.com
<mailto:ishanrathor...@gmail.com>> wrote:

    Dear All,

    I am sorry to disturb you all with this silly question, but this
    problem has been bugging me for quite some time.

    Initially 3 residues were missing in the chain, which I had manually
    tried to build, of which 3 residues from the N-terminus which could
    be easily linked by a peptide bond, while the peptide bond could no
    be formed at the C-terminus (I have attached the pic for the same)
    even though the density is quite clear. I have already tried many
    approaches, including linking of two residues in coot and refining,
    but to no avail.


    Thanks and Regards
    Ishan Rathore





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