It seems your chain ID is different, so it would not connect two different chain ID covalently. So please change your chain ID and try to connect.
Good luck On Sat, May 23, 2015 at 3:53 PM, Ishan Rathore <ishanrathor...@gmail.com> wrote: > Dear All, > > I am sorry to disturb you all with this silly question, but this problem > has been bugging me for quite some time. > > Initially 3 residues were missing in the chain, which I had manually tried > to build, of which 3 residues from the N-terminus which could be easily > linked by a peptide bond, while the peptide bond could no be formed at the > C-terminus (I have attached the pic for the same) even though the density > is quite clear. I have already tried many approaches, including linking of > two residues in coot and refining, but to no avail. > > > Thanks and Regards > Ishan Rathore > > >
