Dera Prerana, Without more information, we cannot help. What have you done, what have you not done, 3.0Å where, etc... Except to say that 3.0Å is not poor at all, often that's all you can get and that's fine. What type of biological or chemical question are you trying to answer ? (we need more info in that sense too). Depending on the question, 3.0Å is enough, and you can refine and get your structure using more or less standard methods.
Cheers, Jose. ================================ Jose Antonio Cuesta-Seijo, PhD Carlsberg Laboratory Gamle Carlsberg Vej 10 DK-1799 Copenhagen V Denmark Tlf +45 3327 5332 Email josea.cuesta.se...@carlsberglab.dk<mailto:josea.cuesta.se...@carlsberglab.dk> ================================ From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Prerana G. Sent: Saturday, April 11, 2015 3:14 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Poor resolution Dear all, I am working on a 40kDa protein. The size and diffraction pattern of the protein crystal seems to be fine but I am getting poor resolution (3.0 Å). How can I increase the resolution? Regards, Prerana