Hi Michael, This depends a bit on how bad your backbone torsion angles are and why. Refining with good restraint weights and flipping the odd peptide can help a lot. If you have really low resolution you might need specific restraints. You can try the PDB_REDO webserver at http://xtal.nki.nl/PDB_REDO which will optimise the restraint weights for REFMAC and flip peptides if the density and the Ramachandran plot show that this is needed. You can also try adding hydrogen bond restrains from ProSMART to help the Ramachandran plot in regions that have reasonable secondary structure. This works quite well at resolutions lower than ~3A.
HTH, Robbie > -----Original Message----- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Michael Murphy > Sent: Wednesday, February 25, 2015 18:09 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] adjusting bad Ramachandran angles > > Does anyone know of a way to adjust Ramachandran angles so that they fall > within the preferred range? Either in Coot or possibly some online server? I > have been trying to do it manually without much success, I was wondering > whether there might another way to do it. -Thanks
