The most important thing is that you do it the same way every time.
But you might also put some thought into flexibility, in case you need
to explore cryoprotectants, etc.
On 11/21/14 11:48, amro selem wrote:
Dear All,
first i wish you nice weekend, then i wanna ask about formulation of
my own mother liquor, i want to optimize a condition containing
0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT)
the question is , should i make a stock solution from every ingredient
by dissolving it in miliQ water. then mix all stuff together and add
water till final concentration with out adjusting final pH. OR
dissolve the PEG in pH adjusted buffer and then mix all ingredients ,
so the final pH will be 8.
any suggest any idea or any one knows what the company do.
CHEERS
Amr
--
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
