Generally one will make this up from stock solutions: Make up 1 M Tris chloride pH 8 1 M magnesium chloride 50 % (weight/volume) PEG 6K
Then mix these together in the right ratios (for 1 mL) 100 uL 1 M tris solution 200 uL 1 M MgCl2 solution 400 uL 50% PEG solution 300 uL water. Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email janet.new...@csiro.au ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of amro selem [000003391c09f749-dmarc-requ...@jiscmail.ac.uk] Sent: 22 November 2014 03:48 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Formulation of my own mother liquor Dear All, first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT) the question is , should i make a stock solution from every ingredient by dissolving it in miliQ water. then mix all stuff together and add water till final concentration with out adjusting final pH. OR dissolve the PEG in pH adjusted buffer and then mix all ingredients , so the final pH will be 8. any suggest any idea or any one knows what the company do. CHEERS Amr
