See also: page 141 in "The Bijvoet-Difference Fourier Synthesis", Jeffrey Roach, METHODS IN ENZYMOLOGY, VOL. 374
Pavel On Fri, Aug 29, 2014 at 12:17 PM, Ronald E Stenkamp < stenk...@u.washington.edu> wrote: > I think the answer is in this paper: LOW-RESOLUTION ELECTRON-DENSITY AND > ANOMALOUS-SCATTERING-DENSITY MAPS OF CHROMATIUM HIGH-POTENTIAL IRON PROTEIN > By: STRAHS, G; KRAUT, J > JOURNAL OF MOLECULAR BIOLOGY Volume: 35 Issue: 3 Pages: 503-& > Published: 1968 > > > > > On Fri, 29 Aug 2014, Alexander Aleshin wrote: > > Could anyone remind me how to calculate anomalous difference Fourier >> maps using model-calculated phases? I was doing it by >> (1) calculating PHcalc from a pdb file using Sfall, then >> (2) merging PHcalc with Dano of experimental SFs, then >> (3) calculating a map with Dano and PHcalc using FFT program of CCP4. >> >> Now, I've read Z. Dauter's et all paper http://mcl1.ncifcrf.gov/ >> dauter_pubs/175.pdf, and it said that their anomalous maps were >> calculated using (delF, PHcalc-90degrees). Why did they use -90 degrees? >> How does it relay to a (delF, phcalc) map? >> >> Thank you for an advice. >> >> Alex Aleshin >> >
