Could anyone remind me how to calculate anomalous difference Fourier maps using model-calculated phases? I was doing it by (1) calculating PHcalc from a pdb file using Sfall, then (2) merging PHcalc with Dano of experimental SFs, then (3) calculating a map with Dano and PHcalc using FFT program of CCP4.
Now, I've read Z. Dauter's et all paper http://mcl1.ncifcrf.gov/dauter_pubs/175.pdf, and it said that their anomalous maps were calculated using (delF, PHcalc-90degrees). Why did they use -90 degrees? How does it relay to a (delF, phcalc) map? Thank you for an advice. Alex Aleshin
