Dear all, we are currently in the process of solving the structure of a Fab/peptide complex. We have first determined the structure of the native Fab and refined it to ~2A, so we have a decent MR model. The complex crystals that we are working on right now diffract to ~3.2A and are unfortunately in P1 (a different spacegroup than the native Fab) with the cell parameters a=70.72 b=91.53 c=206.07 α=95.14 β=99.01 γ=90.83. This large AU contains between 8 and 12 Fab molecules and unfortunaly these are related by pseudo symmetry as suggested by the 4 non-origin distinct peaks detected in the Patterson. Unfortunately this leads to Phaser turning off the pseudotranslation correction and running into one of those endless loops that I stopped eventually after 40 hours. Is there anything we can do in order to get around these different pseudotranslations ?
Thank you so much for any help or suggestions. Best wishes Thomas Dr. Thomas Krey Institut Pasteur Structural Virology Unit 25-28 Rue du Docteur Roux 75015 Paris France tk...@pasteur.fr
