You can prevent them from falling off by also removing 5 microliter or so of mother liquor from the drop and repositioning it back over the reservoir
On Wed, Jul 2, 2014 at 3:38 PM, Patrick Loll <pat.l...@drexel.edu> wrote: > You can cut a small piece of sponge and put that into the reservoir; this > prevents the reservoir buffer from splashing up into the drop. > > The sitting drops should be reasonably safe, but the 10 uL hanging drops > are big; they'll be vulnerable to falling off if the tray is jarred. > > Good luck! > > Pat > > On 2 Jul 2014, at 4:17 PM, Meisam Nosrati wrote: > > > Dear CCP4ers > > > > I need to transfer some crystals mainly in sitting drops to the site of > data collection without freezing them. > > > > I do not know what is the best solution to secure the boxes in their > place to minimize the disturbance. > > > > I am using the 24 well VDX plates with 10-80 microliter sitting drops. I > have one or two hanging drop boxes as well with 10 microliter size drops. > > > > If you have any experience about this matter, I greatly appreciate, if > you share it with me. > > > > Thanks > > > > Meisam Nosrati > > > > > --------------------------------------------------------------------------------------- > Patrick J. Loll, Ph. D. > Professor of Biochemistry & Molecular Biology > Director, Biochemistry Graduate Program > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St., Mailstop 497 > Philadelphia, PA 19102-1192 USA > > (215) 762-7706 > pat.l...@drexelmed.edu >
