Dear Tim, I have used coot.
"merge molecules" (calculate -> merge molecules). Using coot I have moved the molecules to the same asymmetric unit. I have the same problem with two different proteins in one case it works. However, for the other I am still working :-( Greetings, Mônika 2014-02-20 11:09 GMT-03:00 Tim Gruene <t...@shelx.uni-ac.gwdg.de>: > -----BEGIN PGP SIGNED MESSAGE----- > Hash: SHA1 > > Dear Monika, > > would you mind summarising how you solved your problem? It might help > people with similar problems! > > Regards, > Tim > > On 02/20/2014 02:45 PM, Monika Coronado wrote: > > Dear users, > > > > thanks alot for the tips. The problem was solved! > > > > Warmest regard, > > > > > > > > Monika Coronado > > > > > > 2014-02-20 0:57 GMT-03:00 Jens Kaiser <kai...@caltech.edu>: > > > >> Monika, There are several possible causes for the problem you > >> are encountering, but your description is a little too vague to > >> discern them. Scenario 1) You ran phaser with the option "all > >> possible spacegroups", for several different components of your > >> crystal, setup individually, and the runs do not agree on the > >> "best spacegroup"? -- In that case, phaser had problems > >> determining the correct spacegroup, I'd suggest you search for > >> all components, but in separate runs for each possible > >> spacegroup. Scenario 2) You assumed your spacegroup assignment > >> was correct, and ran MR for each of your components individually, > >> and when you display the solutions, they overlap. In this case, > >> you might have your solutions on different origins. The best way > >> out is to use the first solution as a "fixed solution", which is > >> possible in most MR programs, and then search for the next > >> component. There might be other scenarios, if you describe your > >> situation in more detail (how many components in the crystal > >> setup, what program you used, how you used it, and what you mean > >> by "different symmetries"), we might be able to help you better, > >> > >> Cheers, > >> > >> Jens > >> > >> On Tue, 2014-02-18 at 14:59 -0300, Monika Coronado wrote: > >>> Dear, > >>> > >>> Does anyone know how to merge two molecules with different > >>> symmetry? > >>> > >>> I will explain: > >>> > >>> I have done the molecular replacement using the domains of the > >>> molecules separately, now I have to put all together, however > >>> they have a different symmetry. > >>> > >>> > >>> I will appreciate any kind of help. > >>> > >>> > >>> > >>> Regards, > >>> > >>> > >>> > >>> > >>> Mnika > >>> > >>> -- __o _`\<,_ (*)/ (*) -+-+-+-+-+-+- * * * * * * * * * * * * * > >>> * * * * * * * * * * * * * * * * * * * * * * * * * * ...E tudo > >>> muda... > >>> > >>> * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * > >>> * * * * * * * > >>> > >> > >> > >> > > > > > > - -- > - -- > Dr Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > > -----BEGIN PGP SIGNATURE----- > Version: GnuPG v1.4.12 (GNU/Linux) > Comment: Using GnuPG with Icedove - http://www.enigmail.net/ > > iD8DBQFTBgyvUxlJ7aRr7hoRAo2BAJ0VPGwcH9FLrLI09sBwgJL9MPOVWQCgjIy1 > HoLKpUi/ZtXoP5fOOMP/Kso= > =44Yv > -----END PGP SIGNATURE----- > -- __o _`\<,_ (*)/ (*) -+-+-+-+-+-+- * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * ...E tudo muda... * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
