Monika, There are several possible causes for the problem you are encountering, but your description is a little too vague to discern them. Scenario 1) You ran phaser with the option "all possible spacegroups", for several different components of your crystal, setup individually, and the runs do not agree on the "best spacegroup"? -- In that case, phaser had problems determining the correct spacegroup, I'd suggest you search for all components, but in separate runs for each possible spacegroup. Scenario 2) You assumed your spacegroup assignment was correct, and ran MR for each of your components individually, and when you display the solutions, they overlap. In this case, you might have your solutions on different origins. The best way out is to use the first solution as a "fixed solution", which is possible in most MR programs, and then search for the next component. There might be other scenarios, if you describe your situation in more detail (how many components in the crystal setup, what program you used, how you used it, and what you mean by "different symmetries"), we might be able to help you better,
Cheers, Jens On Tue, 2014-02-18 at 14:59 -0300, Monika Coronado wrote: > Dear, > > Does anyone know how to merge two molecules with different symmetry? > > I will explain: > > I have done the molecular replacement using the domains of the > molecules separately, now I have to put all together, however they > have a different symmetry. > > > I will appreciate any kind of help. > > > > Regards, > > > > > Mônika > > -- > __o > _`\<,_ > (*)/ (*) > -+-+-+-+-+-+- > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * > * * * * > ...E tudo muda... > > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * > * * * * >
