Following on from John's comment, when I did my PhD at Birkbeck in the early 2000s, one of David Moss's other PhD students (John Bond) grew some gigantic (>1cm edges) crystals of things like HEWL & Myoglobin, which he then (somewhat perversely) crushed to load into capillaries for powder diffraction analysis.
IIRC, John fashioned a vapour diffusion setup from a 2l Ice cream tub, and used a watch glass to support the sitting drop, which was centimetres across. Truly "bucket crystallography". Regards, Dave ============================ David C. Briggs PhD http://about.me/david_briggs On 24 October 2013 17:08, Jrh <jrhelliw...@gmail.com> wrote: > Dear Tobias, > Take a look at http://dx.doi.org/10.1107/S0108767389012912 > The ribonuclease crystal I used to measure the speed of sound, using laser > generated ultrasound, was of volume 129 mm3 ie 7.7x6.2x2.7 mm . David Moss > of Birkbeck College provided it. > Best wishes, > John > > Prof John R Helliwell DSc FInstP CPhys FRSC CChem F Soc Biol. > Chair School of Chemistry, University of Manchester, Athena Swan Team. > http://www.chemistry.manchester.ac.uk/aboutus/athena/index.html > > > > On 24 Oct 2013, at 16:33, Tobias Beck <tobiasb...@gmail.com> wrote: > > Dear all, > > I was just wondering if anyone has some information or references about > the dimensions of the largest protein crystal ever grown? I am aware that > for neutron protein crystallography one usually needs crystals with mm > dimensions. I have found some information on crystallization under > micro-gravity and how this can enlarge the crystal size. However, I would > rather be interested in the dimensions for crystals obtained from a regular > lab setup. > > Thanks, Tobias. > > -- > ___________________________________________ > > Dr. Tobias Beck > ETH Zurich > Laboratory of Organic Chemistry > Wolfgang-Pauli-Str. 10, HCI F 322 > 8093 Zurich, Switzerland > phone: +41 44 632 68 65 > fax: +41 44 632 14 86 > web: http://www.protein.ethz.ch/people/tobias > ___________________________________________ > >
