Izit is an aqueous solution of methylene blue, which you can prepare simply and cheaply. Look here:
http://www.ysbl.york.ac.uk/ccp4bb/2000/msg00387.html One word of warning: not every crystal stains. I found poking crystal with hair or glass fibre to see if it cracks or breaks is more reliable. Salt crystals react to attacks from glass fibres like rocks (as indeed what they are). From: Swastik Phulera Sent: Tuesday, October 15, 2013 3:59 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Staining Crystals with comassie Dear All, I am looking for a method to quickly differentiate between salt and protein crystals. I have been told thats its a popular alternative to the commercially available izit dye. I would appreciate if some one would share their comassie crystal staining protocol. Swastik
