Yes, and the mosaicity might have been poor to start with, or it might have 
been caused by sub-optimal freezing conditions, assuming that the crystal was 
frozen (not enough information to tell). I recommend doing a room temperature 
diffraction experiment to see which it is and act accordingly - improve the 
crystal or improve the freezing conditions.

Mark

 

 

 

-----Original Message-----
From: Demetres D. Leonidas <ddleoni...@bio.uth.gr>
To: CCP4BB <CCP4BB@JISCMAIL.AC.UK>
Sent: Thu, Oct 3, 2013 7:05 am
Subject: Re: [ccp4bb] X-ray pattern has streaks


Dear Krithika,

what you probably see is the diffraction pattern of a highly mosaic 
crystal due to probably internal disordering.

cheers

Demetres


On 3/10/2013 3:40 μμ, Krithika GOkulnath wrote:
> I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and 
Biophysics, UNiversity of Madras. I have been working with a cloned protein and 
have been successfully able to crystallise it. However data collection done at 
home source shows a pattern of streaks instead of spots. The crystallization 
set 
up contained 12% PEG 8000, 10mM Magnesium chloride, 200mM Potassium chloride in 
50 mM Tris pH 7.0. The protein is an enzyme which is also in the buffer 
containing the above components excepting the precipitant. It is a tetramer at 
native conditions and molecular weight on SDS-PAGE is 54 Kda. Kindly share your 
suggestions and speculations on what might be the reason for the streaking. The 
streaks are many wide spread and there are no high resolution spots or streaks 
as we might expect in salt crystals.

-- 
-----------------------------------------------
Dr. Demetres D. Leonidas
Associate Professor of Biochemistry
Department of Biochemistry & Biotechnology
University of Thessaly
26 Ploutonos Str.
41221 Larissa, Greece
---------------------------------------------
Tel. +302410 565278
Tel. +302410 565297 (Lab)
Fax. +302410 565290
E-mail: ddleoni...@bio.uth.gr
http://www.bio.uth.gr
-----------------------------------------------

 

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