Yes, and the mosaicity might have been poor to start with, or it might have been caused by sub-optimal freezing conditions, assuming that the crystal was frozen (not enough information to tell). I recommend doing a room temperature diffraction experiment to see which it is and act accordingly - improve the crystal or improve the freezing conditions.
Mark -----Original Message----- From: Demetres D. Leonidas <ddleoni...@bio.uth.gr> To: CCP4BB <CCP4BB@JISCMAIL.AC.UK> Sent: Thu, Oct 3, 2013 7:05 am Subject: Re: [ccp4bb] X-ray pattern has streaks Dear Krithika, what you probably see is the diffraction pattern of a highly mosaic crystal due to probably internal disordering. cheers Demetres On 3/10/2013 3:40 μμ, Krithika GOkulnath wrote: > I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and Biophysics, UNiversity of Madras. I have been working with a cloned protein and have been successfully able to crystallise it. However data collection done at home source shows a pattern of streaks instead of spots. The crystallization set up contained 12% PEG 8000, 10mM Magnesium chloride, 200mM Potassium chloride in 50 mM Tris pH 7.0. The protein is an enzyme which is also in the buffer containing the above components excepting the precipitant. It is a tetramer at native conditions and molecular weight on SDS-PAGE is 54 Kda. Kindly share your suggestions and speculations on what might be the reason for the streaking. The streaks are many wide spread and there are no high resolution spots or streaks as we might expect in salt crystals. -- ----------------------------------------------- Dr. Demetres D. Leonidas Associate Professor of Biochemistry Department of Biochemistry & Biotechnology University of Thessaly 26 Ploutonos Str. 41221 Larissa, Greece --------------------------------------------- Tel. +302410 565278 Tel. +302410 565297 (Lab) Fax. +302410 565290 E-mail: ddleoni...@bio.uth.gr http://www.bio.uth.gr -----------------------------------------------