Dear All: I thought that it is possible to be the PEG. The crystallization condition contains 3% of PEG.
Thank you for your comments and input Uma On Thu, May 16, 2013 at 11:58 AM, Mario Sanches <mario...@gmail.com> wrote: > It looks like structured PEG to me. Did you have PEG as a precipitant in > you crystallization solution? This is a quite normal feature, you will find > many examples in the PDB. > > You could try to model it and see how it fits the density, how your > R-factors behave, etc. > > > > > On Thu, May 16, 2013 at 8:45 AM, Uma Ratu <rosiso2...@gmail.com> wrote: > >> Dear All: >> >> I collected a data set (1.6A) of one of my target proteins, and built a >> model by molecular replacement. >> >> I then exam the model by Coot and found two unidentified blobs. The two >> look the same and not in active sites. >> One is close to residues 269 TYR/B and 268 ASN/B, the other is close to >> 25 Gly/B and 67 Pro/B. >> >> The model contains a homo-dimer. The unidentified blobs are only observed >> in one monomer, not in the other one. >> >> I also looked other structures available in this protein family from PDB. >> None has this identified blobs in the position as I observed from my model. >> >> Here is my questions: >> 1. How to identify which compound of this electron density is? >> 2. Because the blobs are not in the active sites, one should just ignore? >> 3. Is it common for unidentified blobs shown in the structure models? >> >> Attached please find the images of the two blobs. Any clue what it is? >> >> Thank you for your comments and advices >> >> Uma >> >> >> > > > > -- > Mario Sanches > http://ca.linkedin.com/in/mariosanches >
