Hi Pascal,
Is this a membrane protein? I worked on a GPCR that gelled upon
high concentration in a detergent/cholesterol solution. It was
reversible upon dilution and ran as a monomer by size exclusion
chromatography. Detergent assays showed that detergent was not being
concentrated in the protein buffer. I was not able to crystallize that
receptor. I like to think we stumbled on some kind of cool receptor
oligomerization phenomenon. I wish I had done more to characterize the
gel instead of cursing the lack of crystals.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
h...@hawaii.edu
