Thank you James, you should write a History book about the modern x-ray times. Or better make one of those movies you are famous for.
Jürgen On Mar 16, 2013, at 10:46 AM, James Holton wrote: The first report of shooting a protein crystal at a synchrotron (I think) was in 1976: http://www.pnas.org/content/73/1/128.full.pdf that was rubredoxin The first PDB file that contains a "SYNCHROTRON=Y" entry is 1tld (trypsin), which was deposited in 1989: http://dx.doi.org/10.1016/0022-2836(89)90110-1 But the structure of trypsin was arguably already "solved" at that time. Anomalous diffraction was first demonstrated by Coster, Knoll and Prins in 1930 http://dx.doi.org/10.1007/BF01339610 this was 20 years before Bijvoet. But not with a synchrotron and definitely not with a protein The first protein to be solved using anomalous was crambin in 1981: http://dx.doi.org/10.1038/290107a0 but this was not using a synchrotron The first demonstration of MAD on a protein at a synchrotron was a Tb soak of parvalbumin in 1985 http://dx.doi.org/10.1016/0014-5793(85)80207-6 but one could argue that several parvalbumins were already known at that time. The first MAD structure from native metals was cucumber blue copper protein (2cbp) in 1989 http://dx.doi.org/10.1126%2Fscience.3406739 The first "new" structure using MAD, as well as the first SeMet was ribonuclease H (1rnh) in 1990 http://dx.doi.org/10.1126/science.2169648 If anyone knows of earlier cases, I'd like to hear about it! -James Holton MAD Scientist On 3/13/2013 7:38 AM, Alan Cheung wrote: Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
