Hi Tim, I only tried HKL2000, and index with different resolution and different number of images. I am not quite familiar with XDS or d*trek.
One thing I am not sure is if this large oscillation angle will cause problem in indexing? If this is true, any method to overcome it? The situation I met was when I chose different settings to do index, HKL2000 would give different cell dimensions while most of them were not small enough for a peptide crystal. The unit cell should be pretty small since even collecting data with 5 degree oscillation angle, the spots in one image were still fewer than a normal protein crystal case, so there is no spots overlap. Best, Yang On Thu, Mar 14, 2013 at 5:20 AM, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: > -----BEGIN PGP SIGNED MESSAGE----- > Hash: SHA1 > > Dear Niu, > > could you let us know more about what you have already tried? > > - - use more images, maybe all images for indexing > - - try a different program: xds instead of mosflm instead of hkl200 > instead of d*trek instead of xds .... depending what you have tried. > - - try to find the unit cell dimensions manually from adxv > - - try cell_now with the SPOTS.XDS > > in XDS I would check the output of IDXREF.LP to figure out if indexing > seems possible, i.e. if the input parameters seem stable or if they > are just floating around > > and many more - it depends on the data set, really! > > Best, > Tim > > On 03/13/2013 09:12 PM, Niu Tou wrote: > > Dear colleagues, > > > > We have some diffraction data from small peptide crystals, the > > shape of diffraction spots looks normal, and resolution is beyond > > 2A. The data were collected with 5 degree rotation per image. Later > > on we found it is hard to do index. Does anybody know some skills > > to figure this problem? > > > > Best wishes, Niu > > > > - -- > - -- > Dr Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > > -----BEGIN PGP SIGNATURE----- > Version: GnuPG v1.4.12 (GNU/Linux) > Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ > > iD8DBQFRQZZFUxlJ7aRr7hoRAmr3AKD28Mml3XY2LIWkDknKrkJFToLDvwCgu1DI > LDaPuAMfGlEIEObuWcckM7Y= > =yuwC > -----END PGP SIGNATURE----- >
