Viswan Try cleaning the supply carboy and lines with bleach. Flush thoroughly with DD water afterwards.
Mark Sent from my iPhone On Mar 8, 2013, at 5:33 PM, "Viswanathan Chandrasekaran" <v...@biochem.utah.edu<mailto:v...@biochem.utah.edu>> wrote: Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don’t have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish