I second that. Gently swirling the bottle (very important) before
pipetting a few microliters (say 100 microliters or whatever).
Dialyse overnight vs 10 mM HEPES 2 mM MgCl2 ph7, then get the protein
concentration to 10 mg/ml. Set up the drops (sitting drops) versus the
same buffer with 30% (v/v) MPD. Direct cryo conditions. Depending on the
material (plastic) used for the sitting drop trays you may find it
difficult to get crystals out - you'll break up a few - but then no
messing up to try to find cryo-conditions.
Fred.
On 04/02/13 17:27, Ganesh Natrajan wrote:
Hi David,
You could try the Glucose Isomerase supplied by Hampton. It
crystallizes under a number of conditions, details of which you can
find in their manual.
http://hamptonresearch.com/product_detail.aspx?cid=28&sid=56&pid=56
Ganesh
--
Fred. Vellieux (B.Sc., Ph.D., hdr)
IBS / ELMA
41 rue Jules Horowitz
F-38027 Grenoble Cedex 01
Tel: +33 438789605
Fax: +33 438785494
