My suggestions would be to look up citations for thaumatin and glucose isomerase. If I remember correctly, both of them form well diffracting crystals within a short period of time. I think you can also buy the purified protein from a vendor. Perhaps you could also try the good old lysozyme.
Cheers, Raji On Mon, Feb 4, 2013 at 11:03 AM, David Roberts <drobe...@depauw.edu> wrote: > So, I know I say this every time I post on this board, but here it goes > again. > > I'm at an undergrad only school, and every 2 years I teach a class in > protein crystallography. This year I'm being super ambitious, and I'm > going to take a class of 16 to the synchrotron for data collection. It's > just an 8 hour thing, to show them the entire process. I'm hoping that we > can collect 5-6 good data sets while there. > > I would like them to grow their own crystals, and go collect data. Then > we'd come back and actually do a molecular replacement (pretty > easy/standard really). Just to get a feel for how it works. > > The protein I do research on is not one that I would push on this, as the > crystals are hard to grow, they are very soft, and the data just isn't the > best (resolution issues). I do have a few that will work on my proteins, > but I was thinking of having others in the class grow up classic proteins > for data collection. Obviously lysozyme is one, but I was wondering what > other standard bulletproof conditions are out there. > > Can you all suggest some protein crystallization conditions (along with > cryo conditions) for some commercially available proteins? I'm looking to > get 6-8 different ones (and we'll just take them and see how it goes). I > wouldn't mind knowing unit cell parameters as well (just a citation works, > I can have them figure it out). I have about 7 weeks to get everything > grown and frozen and ready to go. > > Any help would be greatly appreciated. It always amazes me how helpful > this group is. Thank you very much. > > Dave > -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
