Hi,
First I'm sorry for my blank message earlier.
Doesn't this depend on the oscillation angle? If those images were
collected using 0 to 1° oscillations, I would assume he has a badly
diffracting protein crystal.
Ganesh
Le 13/11/12 11:34, Tim Gruene a écrit :
-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Dear George,
the images don't look like a large cell to me: on the first image you
can see spots from ice rings at about 4A and there are only very few
spots inside that radius, all of which are at beyond 5A.
I'd say there are traces of MgS04 or CaSO4, the Ca or Mg being
left-overs from the expression media.
Regards,
Timd
On 11/13/2012 11:17 AM, George wrote:
Dear colleagues,
There are some crystallographers with (much) more experience than
me.
I ‘ve attached few diffraction images which are not (in my opinion)
typical salt but not typical protein either.
Please let me know you suggestions. Is it worth investigating
further those conditions or there are just salts crystals with
large unit cell.
Attached files:
Crystal.jpg (Photo of crystal)
dif_0.jpg (Diffraction 900 sec exposure/degree at 0 degrees)
dif_90.jpg (Diffraction 900 sec exposure/degree at 90 degrees)
dif_180.jpg (Diffraction 900 sec exposure/degree at 180 degrees)
dif_270.jpg (Diffraction 900 sec exposure/degree at 270 degrees)
protein: 3.3mg/ml in 20 mM TrisHCl pH 8, 150 mM NaCl, 5 mM DTT.
Mother liquor: 28%Jefamine, 0,05mM Li2SO4, 10mM Tris pH 8.0
sitting drops, 1.2ul protein / 1.2ul mother liquor.
Thanks in advance for your help,
George Kontopidis
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf
Of Frank von Delft Sent: Tuesday, November 13, 2012 8:56 AM To:
CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Reservoir buffer
Acta Cryst. (2005). D61, 490-493 [ doi:10.1107/S0907444905002726
<http://dx.doi.org/10.1107/S0907444905002726> ]
Expanding screening space through the use of alternative reservoirs
in vapor-diffusion experiments
J. Newman
<http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Newman,%20J.>
Abstract: Setting up vapor-diffusion crystallization experiments
against four different reservoir solutions showed that the
reservoir solution may have a profound effect on the outcome of a
crystallization experiment. This suggests that a facile way to
increase crystallization space through screening is not to add more
crystallization conditions to the process, but to set up the same
conditions over different reservoirs.
On 13/11/2012 06:03, Theresa Hsu wrote:
Dear all
In *initial screening* using vapor diffusion crystallization, does
it matter whether the reservoir buffer is also the precipitant in
the drop or just a high salt solution like 5 M NaCl?
Thank you.
Theresa
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/
iD8DBQFQoiIZUxlJ7aRr7hoRAlOtAKD9vm5OgG/GH/SMXv4LwTybKnlU3gCghuVH
dFhZk5C5gK3fjVG1z00+jzw=
=VN4A
-----END PGP SIGNATURE-----
