Sorry to disappoint you. It is a diffraction pattern from crystals of salt. Spending some time it is possible to check of which :-\ FF Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 13, 2012, at 12:17 , George <gkontopi...@vet.uth.gr> wrote: > > Dear colleagues, > > There are some crystallographers with (much) more experience than me. > I ‘ve attached few diffraction images which are not (in my opinion) typical > salt but not typical protein either. > Please let me know you suggestions. Is it worth investigating further those > conditions or there are just salts crystals with large unit cell. > Attached files: > Crystal.jpg (Photo of crystal) > dif_0.jpg (Diffraction 900 sec exposure/degree at 0 degrees) > dif_90.jpg (Diffraction 900 sec exposure/degree at 90 degrees) > dif_180.jpg (Diffraction 900 sec exposure/degree at 180 degrees) > dif_270.jpg (Diffraction 900 sec exposure/degree at 270 degrees) > > > protein: 3.3mg/ml in 20 mM TrisHCl pH 8, 150 mM NaCl, 5 mM DTT. > Mother liquor: 28%Jefamine, 0,05mM Li2SO4, 10mM Tris pH 8.0 > sitting drops, 1.2ul protein / 1.2ul mother liquor. > > Thanks in advance for your help, > > George Kontopidis > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Frank > von Delft > Sent: Tuesday, November 13, 2012 8:56 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Reservoir buffer > > > Acta Cryst. (2005). D61, 490-493 [ doi:10.1107/S0907444905002726 ] > > Expanding screening space through the use of alternative reservoirs in > vapor-diffusion experiments > > J. Newman > > Abstract: Setting up vapor-diffusion crystallization experiments against four > different reservoir solutions showed that the reservoir solution may have a > profound effect on the outcome of a crystallization experiment. This suggests > that a facile way to increase crystallization space through screening is not > to add more crystallization conditions to the process, but to set up the same > conditions over different reservoirs. > > > > > On 13/11/2012 06:03, Theresa Hsu wrote: > Dear all > > In *initial screening* using vapor diffusion crystallization, does it matter > whether the reservoir buffer is also the precipitant in the drop or just a > high salt solution like 5 M NaCl? > > Thank you. > > Theresa > > <Crystal.jpg><dif_0.jpg><dif_90.jpg><dif_180.jpg><dif_270.jpg>
