You should and can try, but I guess 5 M NaCl will dry your drop quite fast, unless in your drop NaCl concentration is ~2,5 M :-) That is why first mixture of well and protein solution is 1:1 v/v. Other variations such as 1:9, 9:1 etc sometimes help.
Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 13, 2012, at 08:03 , Theresa Hsu <theresah...@live.com> wrote: > Dear all > > In *initial screening* using vapor diffusion crystallization, does it matter > whether the reservoir buffer is also the precipitant in the drop or just a > high salt solution like 5 M NaCl? > > Thank you. > > Theresa
