I don't know what exactly the phenix.autosol output is. If you are satisfied with the solution and have a mtz file containing F sigF HLA etc, then use Hendrickson Lattmann coefficients.
If there is only Phase/Fom then use that. I am not sure how you would fare with SAD data directly.. But beware - do the HLs etc combine phases from the existing model and the anomalous signal - if so they may be biased towards the existing model, and make it harder to correct Eleanor On 5 Nov 2012, at 09:46, Qixu Cai wrote: > Dear all, > > What's the difference between "no prior phase information", "phase and FOM", > Hendrickson-Lattman coefficients", and "SAD data directly" in the refmac5 GUI > of CCP4i? > > I have a SAD dataset and solve the phase by phenix.autosol. Now I want to > refine the structure by refmac5, which kind of input above I should choose? > > Another question is in the "Refinement Parameters" of refmac5. What's the > meaning of "Use experimental sigmas to weight Xray terms"? If I use molecular > replacement to solve the structure, shall I "uncheck" this item? > > Thanks very much for your help. > > Best wishes, > > Q. Cai >
