Dear Cai
On 5 Nov 2012, at 09:46, Qixu Cai wrote: > Dear all, > > What's the difference between "no prior phase information", "phase and FOM", > Hendrickson-Lattman coefficients", and "SAD data directly" in the refmac5 GUI > of CCP4i? I would use SAD data directly if you have SAD data set. It just means that the program uses observed F+ and F- directly in refinement. This part of the program has been developed by Leiden group so you can direct your questions to them: "Navraj S. Pannu" <r...@chem.leidenuniv.nl> Pavol Skubak <p.sku...@chem.leidenuniv.nl> I am sure they will help you to get started. > > I have a SAD dataset and solve the phase by phenix.autosol. Now I want to > refine the structure by refmac5, which kind of input above I should choose? > > Another question is in the "Refinement Parameters" of refmac5. What's the > meaning of "Use experimental sigmas to weight Xray terms"? If I use molecular > replacement to solve the structure, shall I "uncheck" this item? No. It is related with sigmas of observations and it is desirable to use them. Although there are some question marks how sigmas are estimated, they seemed to be better than nothing. regards Garib > > Thanks very much for your help. > > Best wishes, > > Q. Cai > Dr Garib N Murshudov Group Leader, MRC Laboratory of Molecular Biology Hills Road Cambridge CB2 0QH UK Email: ga...@mrc-lmb.cam.ac.uk Web http://www.mrc-lmb.cam.ac.uk
