Hi, I agree with others here. We produce virus in homemade ISFM and supplement with 2% serum, then store at 4 C protected from light. We don't titer. I have noticed no difference in expression over 1-2 years.
-Tom Cleveland On Sep 27, 2012 8:19 PM, "Vitali Stanevich" <stanev...@wisc.edu> wrote: Alexander, We produce baculovirus at Gibco SF900-II + 10% heated FBS + 1xPSG and then store at 4C routinely. And it seems to be quite stable. We have a virus that had no change for more than 2 years: we judge by the volume of it needed to infect_cells/produce_protein, no titer measurements. We also avoid exposure to light: definitely cover it with foil if it's transparent door fridge. Vitali On Thu, Sep 27, 2012 at 5:42 PM, aaleshin <aales...@burnham.org> wrote: > > Sorry for an off-topic...
