We began experiencing a sudden reduction in stability of baculovirus stock
stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only
difference compared with previous preparations is a switch from Gibco
SF900-II to a media from Lonza (Insect-XPRESS).
Could it be due to the media switch, or something else? What is the best
way to store baculovirus?
Absolutely could be. For long term storage, add 10% FBS. Helps A LOT. I've
used 2 years old stocks stored in serum-containing medium and they worked
fine. We don't titer (because it's kind of pointless) but the optimal virus
to culture volume ratio has not changed more than twoi-fold.
- Dima
