Thank you all for your suggestions. In the same way can Biacore work be done at lower temperature?? I want to do both ITC and Biacore for my protein and peptide regards Rashmi
On Thu, Aug 9, 2012 at 9:19 PM, Edwin Pozharski <epozh...@umaryland.edu>wrote: > > On 08/09/2012 03:55 AM, rashmi panigrahi wrote: > >> Does any one have the experience of doing ITC or Biacore(SPR) at 10 or 15 >> degrees? >> > > You can do ITC at any temperature your instrument allows, certainly no > problems at 4oC. To share a trick, at least on a MIcrocal instrument > cooling down is very slow, and I found it useful to wash the cell with ice > cold water to quickly get down to 4 degrees. > -- rashmi
