On 08/09/2012 03:55 AM, rashmi panigrahi wrote:
Does any one have the experience of doing ITC or Biacore(SPR) at 10 or 15 degrees?
You can do ITC at any temperature your instrument allows, certainly no problems at 4oC. To share a trick, at least on a MIcrocal instrument cooling down is very slow, and I found it useful to wash the cell with ice cold water to quickly get down to 4 degrees.
