My lab has had a C3 for almost 8 years now. It's very good for coli, not so good for yeast as 25-30 kpsi is in the extreme range of the instrument and you'll wear out seals etc pretty quickly. For those pressures you'll also need high pressure air. You'll also need to be fairly disciplined in cleaning, so its not great for sharing between different labs (we all knows how that goes...). Cleaning yourself is possible, but to open it you'll need a big wrench to get sufficient torque. But again, for Ecoli it's very good. We've also had good experiences with the avestin people coming in to do maintenance in terms of waiting time etc.
Bert ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Marcelo Carlos Sousa [marcelo.so...@colorado.edu] Sent: Saturday, August 04, 2012 2:18 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] OFF TOPIC: recommendations for High Pressure Homogenizers Sorry for the off topic question, but it is relevant to those that produce proteins for crystallographic purposes: We are looking to replace an old French Press with a high pressure homogenizer for cell disruption (E. coli and yeast). We are currently looking at the Avestin C3 and the Niro Panda 2000. I would appreciate any feedback (positive or negative) from users of these instruments (or any other competing homogenizer). Thanks in advance Marcelo
