Actually with haeme, and or flavin containing enzymes it is known for the cofactor to undergo reduction. There is a recent JACS paper that explores these changes as a function of X-ray exposure. (2012) J.Am.Chem.Soc. 134: 2823-2834 Excellent work done in part at Allen Orville's X6A As far as quality of your data goes, unless your crystal is suffering from radiation damage (they all do!) from those free electrons/radicals then your data should not be affected by the bleaching. HTH. Yuri
- [ccp4bb] Ferredoxin Containing Crystal Bleaching RHYS GRINTER
- Re: [ccp4bb] Ferredoxin Containing Crystal Bleaching Yuri Pompeu
- Re: [ccp4bb] Ferredoxin Containing Crystal Bleach... Edward A. Berry