Hi All, I was collecting some data at home on a crystal from a protein containing a ferredoxin domain. This crystal was originally red-brown in colour, but after 16h of data collection, a large portion of it appears not be colourless. The crystal still seems to diffract fine (beyond 2A). I assume this it due in some way to the radiation exposure but was wondering out curiosity whether someone had experienced this before and had a specific explanation for it. And if this will effect the integrity of the data.
Cheers Rhys Grinter PhD Candidate University of Glasgow