Dear crystallographers Trying to crystallize a membrane protein complex of 100 kDa with a soluble protein of 20 kDa which is interact with the membrane protein. So far, no co-crystals in > 200 conditions. Some conditions gave crystals but mass spec of crystals show only either one protein present. I am thinking of antibody but don't know where to start. Can I use the anti-His tag antibody?
Thank you.
