I have been using an HRP conjugated antibody from Thermo that can detect maybe 3-8ng of HisTagged protein from insect cell lysates. Performance is ok, but with the sensitivity I am not so happy with.
How is the sensitivity with the Qiagen antibody? How many picograms you need for a clear band? Does anyone know a good HRP conjugated antibody? Regards, Juha On 26 June 2012 06:59, Luca Jovine <luca.jov...@ki.se> wrote: > We routinely use BSA-free anti-5His mAb from QIAGEN, and it works just > fine (at least using media and lysates of both insect and mammalian cells): > > > http://www.qiagen.com/products/protein/detection/qiaexpressdetectionsystem/penta-hisantibody.aspx > > Good luck, > > Luca > > ---------------------------------------------------------------- > Luca Jovine, Ph.D. > Assistant Professor & EMBO Young Investigator > Karolinska Institutet > Department of Biosciences and Nutrition & Center for Biosciences > Hälsovägen 7, SE-141 83 Huddinge, Sweden > Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501 > E-mail: luca.jov...@ki.se > W3: http://jovinelab.org > ---------------------------------------------------------------- > > ________________________________________ > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of D Bonsor [ > dbon...@ihv.umaryland.edu] > Sent: Monday, June 25, 2012 23:33 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Off-topic His-Antibody > > Are there any good antibodies for His-tags on the market. I have never > used one and I heard several stories that they were poor and not worth the > money, over the past few years. The only post I could find on the BB was > from 2008. Have His-Antibodies improved or are they still rubbish. > > > > -- "Genius may have its limitations, but stupidity is not thus handicapped." -Elbert Hubbard *
