The one we have is rubbish. I can tell a vendor name if you want off-list. A lot of non-specific bands + high background level makes it almost impossible to use against cell lysate. It sort of works against purified His-tag protein, but not perfect either.
On Mon, Jun 25, 2012 at 4:33 PM, D Bonsor <dbon...@ihv.umaryland.edu> wrote: > Are there any good antibodies for His-tags on the market. I have never > used one and I heard several stories that they were poor and not worth the > money, over the past few years. The only post I could find on the BB was > from 2008. Have His-Antibodies improved or are they still rubbish. >
