Yes, I had several cases like that - at least two published (PTPbeta and the TurboGFP structures). In the first case the protein needed a touch of beta-OG to stay soluble after the MBP tag was cut off (you have to wonder how many folks tried cutting off MBP, noticed that the product crashes and decided to stop). In the second case detergents gave crystals and two detergents (CYMAL and C-HEGA) gave different crystal forms (with neither detergent clearly visible in density).
Try as many of them as you can afford (protein constraints?) Artem On Thu, Jun 14, 2012 at 9:35 PM, wu donghui <wdh0...@gmail.com> wrote: > Dear all, > > I wonder if anyone has successful experience on using detergent to > crystallize non-membrane protein. Based on what criteria to choose detergent > to help solubilize and crystallize your non-membrane protein. Thanks for any > input or comments. > > Best regards, > > Donghui
