if you have "peek" surface or titanium parts (if i recall right) there are no problems with salt solutions.
tommi On May 30, 2012, at 3:39 AM, aaleshin wrote: > Back in Iowa State University we used Waters HPLC for protein purification > during many years without noticeable damage to the stainless steel tubings. > But Dan was right about the pumps, someone in the lab forgot to flush the > high salt pump with water after its use and damaged the pump... > > Alex > > On May 29, 2012, at 5:14 PM, Daniel Anderson wrote: > >> Hi, Ho, >> >> Your question has a lot of variables. >> >> "HPLC" columns should not be used on the Akta within my field of view >> because the Akta within my field of view does not have gradual pump >> acceleration and deceleration. "HPLC" columns can be damaged by sudden >> changes in pressure or composition. >> >> The "HPLC" within my field of view has wetted stainless steel surfaces >> and the mobile phase should not contain chloride ion or reductant. >> Chloride ion would accelerate corrosion of the stainless steel (and >> result in metal ions in the protein). Reductant would strip off the >> "passivation" (during maintenance I soak the stainless parts in nitric >> acid to keep them stainless) later resulting in corrosion. >> >> The Waters sales representative once told me that the pumps have to be >> salt-free and methanol-flushed at the end of every working day. Good >> luck implementing that policy. >> >> -Dan >> >> Ho Leung Ng wrote: >>> Hello, >>> >>> My Akta Purifier is being repaired, and I'm thinking about >>> borrowing a colleague's HPLC in the interim. What makes the Aktas >>> different from HPLCs? I've used HPLCs for purifying small molecules >>> and peptides but not proteins. Anything I should be careful about >>> regarding keeping the machines, columns, and proteins happy? >>> >>> >>> Thank you, >>> Ho >>> >>> Ho Leung Ng >>> University of Hawaii at Manoa >>> Assistant Professor, Department of Chemistry >>> h...@hawaii.edu <mailto:h...@hawaii.edu>
