Hi, Ho,
Your question has a lot of variables.
"HPLC" columns should not be used on the Akta within my field of view
because the Akta within my field of view does not have gradual pump
acceleration and deceleration. "HPLC" columns can be damaged by sudden
changes in pressure or composition.
The "HPLC" within my field of view has wetted stainless steel surfaces
and the mobile phase should not contain chloride ion or reductant.
Chloride ion would accelerate corrosion of the stainless steel (and
result in metal ions in the protein). Reductant would strip off the
"passivation" (during maintenance I soak the stainless parts in nitric
acid to keep them stainless) later resulting in corrosion.
The Waters sales representative once told me that the pumps have to be
salt-free and methanol-flushed at the end of every working day. Good
luck implementing that policy.
-Dan
Ho Leung Ng wrote:
Hello,
My Akta Purifier is being repaired, and I'm thinking about
borrowing a colleague's HPLC in the interim. What makes the Aktas
different from HPLCs? I've used HPLCs for purifying small molecules
and peptides but not proteins. Anything I should be careful about
regarding keeping the machines, columns, and proteins happy?
Thank you,
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
h...@hawaii.edu <mailto:h...@hawaii.edu>
