On Sat, May 5, 2012 at 12:17 PM, ruby singh <singh.rub...@gmail.com> wrote:
> Sir, > i m sorry for not giving full details of protein. > its plant lectin protein on which im working currently. its sequence > information is very little. crystallization has become near to impossible. > the protein is a tetramer( of 28KDa). its purification is very > simple...but for crystallization i tried many things nt working > though(Hampton Reserach-pegion and index/CSS1/JCSG etc..). need some help. > the protein conecentration used was 30mg/ml stock . most of the > crystallization condition shows precipitation. the set up was put at 20C. > the protein is very stable in MilliQ water upto 100mg/ml concentration with > no precipitation. > the protein doesnt aggregate..doesnt loose activity even after boiling at 80C. getting its crystal is important for my thesis. so need help.
