Dear Dipankar,
It just occurred to me that your high Rfactors may also be due to a
large conformational change of your protein. In that case you have to
split your search model in separate pdb files for the separate domains
and rund Molrep with these separate domains.
Best,
Herman
________________________________
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Behalf Of Dipankar Manna
Sent: Wednesday, March 14, 2012 10:26 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How to reduce R factor
Dear Crystallographers,
Can anybody guide me how to reduce R-factor, means which are the
basic parameters I have to look for to reduce the R-factor during
refinement. I am newly learning the refinement. After running molrep
R-factor is around 53% (100% identity), after rigid body refinement its
showing around 49% and after restrained refinement its showing around
47%. Highest resolution is 2.5A.
Regards
Dipankar
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