-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Dipankar,
if you refine your model straight after molecular replacement you risk to further strengthen model bias which could result in hovering out features in your data which otherwise help you improve your model. Look at the model and the map after rigid body refinement with the model building program of your choice and improve the model as much as you can before you run any further refinement. If you do not see any features in the map deviating from the model chances are high that your MR solution is incorrect. Best wishes, Tim On 03/14/2012 10:26 AM, Dipankar Manna wrote: > Dear Crystallographers, > > Can anybody guide me how to reduce R-factor, means which are the basic > parameters I have to look for to reduce the R-factor during refinement. I am > newly learning the refinement. After running molrep R-factor is around 53% > (100% identity), after rigid body refinement its showing around 49% and after > restrained refinement its showing around 47%. Highest resolution is 2.5A. > > Regards > > Dipankar > > ________________________________ > > This e-mail and any files transmitted with it are for the sole use of the > intended recipient(s) and may contain confidential and privileged > information.If you are not the intended recipient, please contact the sender > by reply e-mail and destroy all copies of the original message.Any > unauthorized review, use, disclosure, dissemination, forwarding,printing or > copying of this email or any action taken in reliance on this e-mail is > strictly prohibited and may be unlawful. > > Visit us at http://www.aurigene.com > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.10 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFPYGUbUxlJ7aRr7hoRArrAAKCpS31k0HNopSponGGZ52i4nYwxZgCfWrN2 1+T+EvfljKRiKf3Q8DfosMk= =qZ5E -----END PGP SIGNATURE-----
