Min-Kyu, This sounds like to be a hydrophobicity ~ temperature issue (as Kevin pointed out, pH gets involved too).
If your protein is sensitive in this regards, it could form different oligomerization states at diff temp, associated with diff solubility. You may want to crystallize it at diff temperatures. Lijun On Mar 12, 2012, at 11:02 AM, Min-Kyu Cho wrote: > Hi all, > > I have a homotetrameric coiled-coil domain sample with 45aa per each. While I > store this sample at 4oC, the sample looks clear w/o any particles. But when > I took out the sample to my bench at r.t, I can see there are precipitates > (as stack of needle like particles) at the bottom of the tube after several > hours. Interestingly, when I put it back into 4oC fridge, the precipitates > disappeared and the solution turned into clear again. > > Does anyone have knowledge of such behavior of any protein? I appreciate any > information related. > > Min-Kyu
