Which kind of buffer you use? If it is Tris, then temperature change will cause pH change.
Actually, this is a good way for crystallization. Kevin On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <min-kyu....@live.com> wrote: > Hi all, > > I have a homotetrameric coiled-coil domain sample with 45aa per each. While > I store this sample at 4oC, the sample looks clear w/o any particles. But > when I took out the sample to my bench at r.t, I can see there are > precipitates (as stack of needle like particles) at the bottom of the tube > after several hours. Interestingly, when I put it back into 4oC fridge, the > precipitates disappeared and the solution turned into clear again. > > Does anyone have knowledge of such behavior of any protein? I appreciate any > information related. > > Min-Kyu
