On 01/03/12 14:26, Uma Ratu wrote:
Hello,
I run my model in Coot to do "Temp Fact Variance Analysis".
There are red bars from the B-factor Variance graphy.
I click each red bar to exam the residues in Coot. Many of these
residues do not have the electronic density on their side chains,
especially Lys residues.
Here is my questions:
1. The lack of electronic density is the cause of these red-bars?
2. How do I fix them? delete the side chains?
The diffraction data is 2A, and data completness is 98.8%.
I'd think twice about "fixing" these red bars.
The signal to noise in the temperature factor variance graphs are
unimpressively low. Currently red bars indicate "there might be
something wrong here" not "there is something wrong here".
Paul.
