Quote"Particulalrly the monoolein and monoolein/cholesterol coated plates ( I am not sure I can mention the vendor here but it "should" not matter)" Since the person who asked this question here forget about it alltogether to write something back here is what he was asking about (i think)
Anybody heard about the nextal plates see the link http://www.qiagen.com/products/nextalcubicphaseproducts.aspx It uses a different method of mixing lipids other than using the syringe method. They have lipid layered on the bottom of the well and protein samples are pipetted onto the monoolein.and later on precipitant. Plastic based ones are really bad idea Not everyone have an access to UV even if UV screening is used not a full-proof method to detect membrane protein micro crystals I would definitely think glass based plates are the way to go. On Sat, Feb 25, 2012 at 3:35 PM, Yuri Pompeu <yuri.pom...@ufl.edu> wrote: > Hello Everyone, > I am considering the purchase of crystallization plates for membrane proteins. > I would love to hear what some of the community thinks or has experienced > with these. > Particulalrly the monoolein and monoolein/cholesterol coated plates ( I am > not sure I can mention the vendor here but it "should" not matter) > So fire away. Is it worth it? Any succes stories? Bad experiences? > I appreciate the input > Best, > Yuri -- Pius S Padayatti,PhD, Phone: 216-658-4528
