Hi board, Although i do not want to endorse anything personally earlier mail to Yuris response reflects my personal reflection of what i like.
Here is a plate from Molecualr dimensions that i found may be an alternate where you can chose the sandwich thickness good idea but do not know what advantage it might have on crystallization process. link: http://www.moleculardimensions.com//shopdisplayproducts.asp?Search=Yes Thanks to Tony from molecular dimensions who point this out. quote: "We have many users of Laminex which offers a lot of flexibility in that you can choose sandwich thickness as well as glass or plastic bases and glass, plastic or film covers. If you have not tested Laminex I would be pleased to send you a sample trial pack. Tony Savill Molecular Dimensions Inc." -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Pius Padayatti Sent: 26 February 2012 01:08 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Membrane Protein Crystallization Plates Hi Yuri, i strongly suggest these plates sold through Hampton research Paul Marienfeld GmbH plates for your use. here is a link , best plates in the market. link: http://hamptonresearch.com/product_detail.aspx?cid=10&sid=182&pid=611 Plates comes with extra cover slips in addition to single glass cover for four wells at a time whcih let you set these plates manually if you like it that way. Good for automations as well. only problem: since the galss is siliconized you can not write anything on it (may be there is some industry grade marker might work.) But there is enough place on side to stick a printed label or bar code for your'automation for keeping records hope this is what you wanted to know. if you are money crunched and want to make some plates on your own. buy regular micoscope slides, some two sided tapes (3M) very thin and a tool that sold for cutting out small pieces of tissue samples. it looks like a pen with sharp punch enough to make a hole in a 3M tape make a 27 hole plate in a standard microslide. cheers pius On Sat, Feb 25, 2012 at 3:35 PM, Yuri Pompeu <yuri.pom...@ufl.edu> wrote: > Hello Everyone, > I am considering the purchase of crystallization plates for membrane proteins. > I would love to hear what some of the community thinks or has experienced with these. > Particulalrly the monoolein and monoolein/cholesterol coated plates ( > I am not sure I can mention the vendor here but it "should" not matter) So fire away. Is it worth it? Any succes stories? Bad experiences? > I appreciate the input > Best, > Yuri -- Pius S Padayatti,PhD, Phone: 216-658-4528 -- Pius S Padayatti,PhD, Phone: 216-658-4528
