Our recombinant product is formulated in 10 mM sodium acetate buffer at pH 4.0 and the std composition mentions sodium 0.035 mg and acetate 0.59 mg per ml of sample. It mentions Sodium acetate is formed by titrating glacial acetic acid with sodium hydroxide.
Can anyone guide me on how this 10 mM buffer is prepared or on its calculation. What currently I do is the old method that we have been following i.,e Use 0.123 mg Na Acetate trihydrate + 0.476 µl Gl. Acetic acid, adjust pH to 4.0 using 5 N NaOH. But we do not know how these values have been arrived at and if this calculation is correct. Please guide me with this regards. Any help on this will be highly appreciated. thanks, Megha
